Testing for macromolecules

Students draw four squares onto their brown paper bag, then use a cotton swab to put samples of three lipids of their choice and water as a control into the squares. Lipids consist of glycerol and fatty acids "tails". Each nucleotide is made of a sugar molecule, a phosphate molecule and a nitrogenous base.

Then repeat with a test tube containing only water. For the slide that is added with aceto-orcein stain, the nucleus of the cell can be seen as it is stained by the Testing for macromolecules stain.

Testing for Macromolecules

Are long chains of monosaccharides bond together. Hair, fingernails, muscles, tendons, cartilage, enzymes, antibodies, hemoglobin, hormones, etc. Sudan Red test Sudan red is a fat-soluble dye that stains lipids red.

The different amino acids are similar in structure: When distilled water was added to the test tube, cloudiness also occurred. D Test for proteins The cooked egg white was still in solid form when sodium hydroxide was added to it.

This can best Testing for macromolecules seen when you hold the paper up to a light source.

Put ml of corn syrup in a test tube Add 1ml of the Benedict Reagent, the solution will look blue Put the tube in a gently boiling water bath. Checking for student understanding: If the material is solid tell students to chop it finely or mush it in a mortar and add distill water before adding the reagent.

Refer to page 17 and 18 Stage 2 Biology Results: Lipids A lot of lipids function as long-term energy storage. Solid fats are mostly animal derived. Lipids are characterized by being water-insoluble but soluble in nonpolar organic solvents.

B Lipids Lipids are one class of aliphatic hydrocarbon-containing organic compounds essential for the structure and function of living cells. This lesson can easily tight into a health and nutrition unit.

Proteins also play an important part in the immune system antibodiesoxygen transport hemoglobinmovement muscles etc. Testing for proteins Buiret test Buiret solution is a blue liquid that changes to purple when proteins are present and to pink in the presence of short chains of polypeptides.

In RNA ribose nucleic acid the sugar is a ribose and the bases are adenine, guanine, cytosine and uracil. This blue spots starch granules indicates that potato contains carbohydrate. Nutrition To test for macromolecules carbohydrates, lipids, nucleic acids, and proteinsiodine solution, alcohol, aceto-orcein stain and copper sulfate solution are used.

For older students provide more detail: Show students the positive - purple or pink - test result indicating the presence of protein.

When distilled water was added to the test tube, cloudiness occurred. Students will see that Sudan red with stain the fat molecules. Monosaccharides or simple sugars such as glucose and fructose C6H12O6 function as energy source in cells during cellular respiration and are also used to build cell structures and other organic molecules within the cells.

Biuret reagent can stain your skin and fingernails!! The depth of the scientific content discussed with the students will depend on their age and the lesson focus for ex.

Also roaming the classroom, watching students perform their tests, and asking probing questions also will provide valuable information about their understanding of the subject. Then add drops of Sudan red to the mix. They contain a higher number of unsaturated fatty acids which have "bends" in the fatty acid chain due to double bonds between the carbon atoms.

Demonstrate to students how to perform the test. The seeds are loaded with oil and will leave behind grease spots.

Testing for Lipids, Proteins and Carbohydrates

Discuss the importance of a control. Disaccharides are composed of two monosaccharides joined together. Nucleic acids are found in all living cells and viruses.

Daly Ralston Resource Center: This affected the image that was viewed under the microscope. Examples of lipids are fats and oils.3) For test tube 2, place 2 ml of Solution 1 into test tube, follow the previously listed procedure for detection of Starch starting at Step 3.

4) For test tube 3, place 2 ml of Solution 1 into test tube, follow the previously listed procedure for detection of Proteins starting at Step 3.

5) Record your findings for Solution 1 in the table below. Testing for macromolecules. Testing for carbohydrates. Testing for the presence of starch (complex sugar) Lugol's reagent (iodine solution) changes from yellowish-brown to dark purple/black. Testing for simple carbohydrates (monosaccharides and some disaccharides) Benedict's solution is used to test for simple carbohydrates.

Benedict's. BG: Lugol's Reagent, or iodine solution, is used to test for the presence of starch. Starch's long coil structure has a hole through the middle that perfectly complements iodine molecules. To test, add drop of iodine solution into substance being tested.

To test for macromolecules (carbohydrates, lipids, nucleic acids, and proteins), iodine solution, alcohol, aceto-orcein stain and copper sulfate solution are used. The conclusions for all of the tests are positive.

Start studying Testing for Macromolecules. Learn vocabulary, terms, and more with flashcards, games, and other study tools. 1. Using masking tape label a set of test tubes with A to G.

2. Add about 10 drops of each test solution to the appropriate test tube according to Table 1, then add ml of the Benedict’s solution to each test tube.

Mix sample thoroughly before transfer it .

Testing for macromolecules
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